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human fetal lung diploid fibroblastic cells  (ATCC)


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    ATCC human fetal lung diploid fibroblastic cells
    Human Fetal Lung Diploid Fibroblastic Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fetal lung diploid fibroblastic cells/product/ATCC
    Average 99 stars, based on 962 article reviews
    human fetal lung diploid fibroblastic cells - by Bioz Stars, 2026-03
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    KB1541 ameliorates senescence phenotypes. ( A ) Electron microscopy analysis revealed that DMSO-treated senescent fibroblasts exhibited less cristae wrinkle folds (blue arrow), whereas KB1541 treatment in senescent fibroblasts showed increased the number of cristae wrinkle folds (red arrow). Upper image: Scale bar 2 μm. Lower image: Scale bar 200 nm. ( B ) Comparison of cristae length between DMSO and KB1541-treated senescent fibroblasts. * P < 0.05, student t -test. Mean ± S.D., n = 6. ( C and D ) Flow cytometric analysis of ROS and mitochondrial mass using MitoSOX and MitoTracker green, respectively. ** P < 0.01, student t -test. Mean ± S.D., n = 3. Flow cytometry data of ROS and mitochondrial mass were presented in . ( E ) Flow cytometric analysis of autophagy level using Cyto–ID assay. ** P < 0.01, student t -test. Mean ± S.D., n = 3. Flow cytometric data of autophagy level were presented in . ( F ) Quantification of SA-β gal positive cells. ** P < 0.01, student t -test. Means ± S.D., n = 4. Scale bar 100 μm.

    Journal: Aging (Albany NY)

    Article Title: Targeting regulation of ATP synthase 5 alpha/beta dimerization alleviates senescence

    doi: 10.18632/aging.203858

    Figure Lengend Snippet: KB1541 ameliorates senescence phenotypes. ( A ) Electron microscopy analysis revealed that DMSO-treated senescent fibroblasts exhibited less cristae wrinkle folds (blue arrow), whereas KB1541 treatment in senescent fibroblasts showed increased the number of cristae wrinkle folds (red arrow). Upper image: Scale bar 2 μm. Lower image: Scale bar 200 nm. ( B ) Comparison of cristae length between DMSO and KB1541-treated senescent fibroblasts. * P < 0.05, student t -test. Mean ± S.D., n = 6. ( C and D ) Flow cytometric analysis of ROS and mitochondrial mass using MitoSOX and MitoTracker green, respectively. ** P < 0.01, student t -test. Mean ± S.D., n = 3. Flow cytometry data of ROS and mitochondrial mass were presented in . ( E ) Flow cytometric analysis of autophagy level using Cyto–ID assay. ** P < 0.01, student t -test. Mean ± S.D., n = 3. Flow cytometric data of autophagy level were presented in . ( F ) Quantification of SA-β gal positive cells. ** P < 0.01, student t -test. Means ± S.D., n = 4. Scale bar 100 μm.

    Article Snippet: Human diploid fibroblasts (HDF; PCS–201–010; ATCC, Manassas, VA, USA) and Human embryonic kidney cells (HEK293T; CRL–11268; ATCC) were used in this study.

    Techniques: Electron Microscopy, Comparison, Flow Cytometry

    Maintenance of 14–3–3ζ expression is a prerequisite for KB1541-mediated senescence alleviation. ( A ) Expression levels of 14–3–3ζ and ATP synthase 5 alpha/beta proteins in young and senescent fibroblasts. ( B ) shRNA-mediated 14–3–3ζ knockdown significantly reduced the endogenous expression level of 14–3–3ζ mRNA. ** P < 0.01, student t -test. Mean ± S.D., n = 3. ( C ) Immunoblot of total lysates from senescent cells transduced with lenti-virus expressing control shRNA or 14–3–3ζ shRNA. ( D ) Quantification of SA-β gal positive cells. * P < 0.05, student t -test. Mean ± S.D., n = 3.

    Journal: Aging (Albany NY)

    Article Title: Targeting regulation of ATP synthase 5 alpha/beta dimerization alleviates senescence

    doi: 10.18632/aging.203858

    Figure Lengend Snippet: Maintenance of 14–3–3ζ expression is a prerequisite for KB1541-mediated senescence alleviation. ( A ) Expression levels of 14–3–3ζ and ATP synthase 5 alpha/beta proteins in young and senescent fibroblasts. ( B ) shRNA-mediated 14–3–3ζ knockdown significantly reduced the endogenous expression level of 14–3–3ζ mRNA. ** P < 0.01, student t -test. Mean ± S.D., n = 3. ( C ) Immunoblot of total lysates from senescent cells transduced with lenti-virus expressing control shRNA or 14–3–3ζ shRNA. ( D ) Quantification of SA-β gal positive cells. * P < 0.05, student t -test. Mean ± S.D., n = 3.

    Article Snippet: Human diploid fibroblasts (HDF; PCS–201–010; ATCC, Manassas, VA, USA) and Human embryonic kidney cells (HEK293T; CRL–11268; ATCC) were used in this study.

    Techniques: Expressing, shRNA, Knockdown, Western Blot, Transduction, Virus, Control